100 ng Search Results


quality control  (Zymo Research)
94
Zymo Research quality control
Quality Control, supplied by Zymo Research, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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zymo control  (Zymo Research)
90
Zymo Research zymo control
Zymo Control, supplied by Zymo Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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quality control data  (Zymo Research)
91
Zymo Research quality control data
Quality Control Data, supplied by Zymo Research, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
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control ev transfected protoplasts  (Zymo Research)
94
Zymo Research control ev transfected protoplasts
Control Ev Transfected Protoplasts, supplied by Zymo Research, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
control ev transfected protoplasts - by Bioz Stars, 2026-03
94/100 stars
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growth factors 3l 100 ng/ml  (Immunex Corporation)
90
Immunex Corporation growth factors 3l 100 ng/ml
Growth Factors 3l 100 Ng/Ml, supplied by Immunex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
growth factors 3l 100 ng/ml - by Bioz Stars, 2026-03
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trail (solvent, 0.1% bovine serum albumin [bsa] to a concentration of 100 ng/μl)  (PeproTech)
90
PeproTech trail (solvent, 0.1% bovine serum albumin [bsa] to a concentration of 100 ng/μl)
Trail (Solvent, 0.1% Bovine Serum Albumin [Bsa] To A Concentration Of 100 Ng/μl), supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
trail (solvent, 0.1% bovine serum albumin [bsa] to a concentration of 100 ng/μl) - by Bioz Stars, 2026-03
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noggin 200 ng/ml  (PeproTech)
90
PeproTech noggin 200 ng/ml
Noggin 200 Ng/Ml, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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100 ng/ml rmuifn-g  (Kishida Chemical)
90
Kishida Chemical 100 ng/ml rmuifn-g
100 Ng/Ml Rmuifn G, supplied by Kishida Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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100 ng ifn-γ  (Schering-Plough corporation)
90
Schering-Plough corporation 100 ng ifn-γ
Concentration and activity of IL-10 secreted by IL-10-transfected B16-melanoma cell lines. The B16-melanoma cell line was transfected with IL-10 cDNA and the production of IL-10 was quantified from cell culture supernatants by ELISA. Three clones that secreted high (B16-10), medium (B16-13) and low (B16-5) amounts of IL-10 were selected. Results are expressed in pg/ml of IL-10/2 × 106 cells/48 hr. The biological activity of the secreted IL-10 was determined by measuring the nitrite (NO2) concentrations after macrophages were incubated with cell line supernatants and then activated by <t>LPS/IFN-γ</t> (see Material and Methods). Labels at the left of the slash indicate the cell-line supernatant and the sign ‘−’ indicates not incubated with cell-line supernatant; ‘+’ or ‘−’ signals at the right of the slash indicate activation or not activation of macrophages. The bar representing the nitrite basal production by macrophages is indicated as ‘−/−’ (no cell line supernatant/not activated) and total nitrite production after stimulation is indicated as −/+ (no cell line supernatant/activated). The mean of triplicates is presented and error bars represent the standard errors of means.
100 Ng Ifn γ, supplied by Schering-Plough corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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murine soluble tnfsf11  (PeproTech)
90
PeproTech murine soluble tnfsf11
Concentration and activity of IL-10 secreted by IL-10-transfected B16-melanoma cell lines. The B16-melanoma cell line was transfected with IL-10 cDNA and the production of IL-10 was quantified from cell culture supernatants by ELISA. Three clones that secreted high (B16-10), medium (B16-13) and low (B16-5) amounts of IL-10 were selected. Results are expressed in pg/ml of IL-10/2 × 106 cells/48 hr. The biological activity of the secreted IL-10 was determined by measuring the nitrite (NO2) concentrations after macrophages were incubated with cell line supernatants and then activated by <t>LPS/IFN-γ</t> (see Material and Methods). Labels at the left of the slash indicate the cell-line supernatant and the sign ‘−’ indicates not incubated with cell-line supernatant; ‘+’ or ‘−’ signals at the right of the slash indicate activation or not activation of macrophages. The bar representing the nitrite basal production by macrophages is indicated as ‘−/−’ (no cell line supernatant/not activated) and total nitrite production after stimulation is indicated as −/+ (no cell line supernatant/activated). The mean of triplicates is presented and error bars represent the standard errors of means.
Murine Soluble Tnfsf11, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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100 ng/ml (>500 u/ml) interleukin-4  (PeproTech)
90
PeproTech 100 ng/ml (>500 u/ml) interleukin-4
Concentration and activity of IL-10 secreted by IL-10-transfected B16-melanoma cell lines. The B16-melanoma cell line was transfected with IL-10 cDNA and the production of IL-10 was quantified from cell culture supernatants by ELISA. Three clones that secreted high (B16-10), medium (B16-13) and low (B16-5) amounts of IL-10 were selected. Results are expressed in pg/ml of IL-10/2 × 106 cells/48 hr. The biological activity of the secreted IL-10 was determined by measuring the nitrite (NO2) concentrations after macrophages were incubated with cell line supernatants and then activated by <t>LPS/IFN-γ</t> (see Material and Methods). Labels at the left of the slash indicate the cell-line supernatant and the sign ‘−’ indicates not incubated with cell-line supernatant; ‘+’ or ‘−’ signals at the right of the slash indicate activation or not activation of macrophages. The bar representing the nitrite basal production by macrophages is indicated as ‘−/−’ (no cell line supernatant/not activated) and total nitrite production after stimulation is indicated as −/+ (no cell line supernatant/activated). The mean of triplicates is presented and error bars represent the standard errors of means.
100 Ng/Ml (>500 U/Ml) Interleukin 4, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/100 ng/ml (>500 u/ml) interleukin-4/product/PeproTech
Average 90 stars, based on 1 article reviews
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90/100 stars
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methocult medium containing il-3 (2.5 ng/ml) scf (100 ng/ml  (PeproTech)
90
PeproTech methocult medium containing il-3 (2.5 ng/ml) scf (100 ng/ml
Concentration and activity of IL-10 secreted by IL-10-transfected B16-melanoma cell lines. The B16-melanoma cell line was transfected with IL-10 cDNA and the production of IL-10 was quantified from cell culture supernatants by ELISA. Three clones that secreted high (B16-10), medium (B16-13) and low (B16-5) amounts of IL-10 were selected. Results are expressed in pg/ml of IL-10/2 × 106 cells/48 hr. The biological activity of the secreted IL-10 was determined by measuring the nitrite (NO2) concentrations after macrophages were incubated with cell line supernatants and then activated by <t>LPS/IFN-γ</t> (see Material and Methods). Labels at the left of the slash indicate the cell-line supernatant and the sign ‘−’ indicates not incubated with cell-line supernatant; ‘+’ or ‘−’ signals at the right of the slash indicate activation or not activation of macrophages. The bar representing the nitrite basal production by macrophages is indicated as ‘−/−’ (no cell line supernatant/not activated) and total nitrite production after stimulation is indicated as −/+ (no cell line supernatant/activated). The mean of triplicates is presented and error bars represent the standard errors of means.
Methocult Medium Containing Il 3 (2.5 Ng/Ml) Scf (100 Ng/Ml, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
methocult medium containing il-3 (2.5 ng/ml) scf (100 ng/ml - by Bioz Stars, 2026-03
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Image Search Results


Concentration and activity of IL-10 secreted by IL-10-transfected B16-melanoma cell lines. The B16-melanoma cell line was transfected with IL-10 cDNA and the production of IL-10 was quantified from cell culture supernatants by ELISA. Three clones that secreted high (B16-10), medium (B16-13) and low (B16-5) amounts of IL-10 were selected. Results are expressed in pg/ml of IL-10/2 × 106 cells/48 hr. The biological activity of the secreted IL-10 was determined by measuring the nitrite (NO2) concentrations after macrophages were incubated with cell line supernatants and then activated by LPS/IFN-γ (see Material and Methods). Labels at the left of the slash indicate the cell-line supernatant and the sign ‘−’ indicates not incubated with cell-line supernatant; ‘+’ or ‘−’ signals at the right of the slash indicate activation or not activation of macrophages. The bar representing the nitrite basal production by macrophages is indicated as ‘−/−’ (no cell line supernatant/not activated) and total nitrite production after stimulation is indicated as −/+ (no cell line supernatant/activated). The mean of triplicates is presented and error bars represent the standard errors of means.

Journal:

Article Title: Interleukin-10 promotes B16-melanoma growth by inhibition of macrophage functions and induction of tumour and vascular cell proliferation

doi: 10.1046/j.1365-2567.2002.01363.x

Figure Lengend Snippet: Concentration and activity of IL-10 secreted by IL-10-transfected B16-melanoma cell lines. The B16-melanoma cell line was transfected with IL-10 cDNA and the production of IL-10 was quantified from cell culture supernatants by ELISA. Three clones that secreted high (B16-10), medium (B16-13) and low (B16-5) amounts of IL-10 were selected. Results are expressed in pg/ml of IL-10/2 × 106 cells/48 hr. The biological activity of the secreted IL-10 was determined by measuring the nitrite (NO2) concentrations after macrophages were incubated with cell line supernatants and then activated by LPS/IFN-γ (see Material and Methods). Labels at the left of the slash indicate the cell-line supernatant and the sign ‘−’ indicates not incubated with cell-line supernatant; ‘+’ or ‘−’ signals at the right of the slash indicate activation or not activation of macrophages. The bar representing the nitrite basal production by macrophages is indicated as ‘−/−’ (no cell line supernatant/not activated) and total nitrite production after stimulation is indicated as −/+ (no cell line supernatant/activated). The mean of triplicates is presented and error bars represent the standard errors of means.

Article Snippet: Then they were stimulated with 10 ng LPS (Sigma Chemical Co.) and 100 ng IFN-γ (Schering-Plough, Mexico City, Mexico) in 500 µl RPMI-1640 for 48 hr.

Techniques: Concentration Assay, Activity Assay, Transfection, Cell Culture, Enzyme-linked Immunosorbent Assay, Clone Assay, Incubation, Activation Assay

Effects of IL-10 on the interactions of immune and tumour cells in vitro

Journal:

Article Title: Interleukin-10 promotes B16-melanoma growth by inhibition of macrophage functions and induction of tumour and vascular cell proliferation

doi: 10.1046/j.1365-2567.2002.01363.x

Figure Lengend Snippet: Effects of IL-10 on the interactions of immune and tumour cells in vitro

Article Snippet: Then they were stimulated with 10 ng LPS (Sigma Chemical Co.) and 100 ng IFN-γ (Schering-Plough, Mexico City, Mexico) in 500 µl RPMI-1640 for 48 hr.

Techniques: Lysis, Expressing